Cell Growth Media and Protein Production
Preparing isotope-enriched protein using cell-based expression systems is the most popular method to prepare samples for NMR analysis. By far, the most popular organism used to express labeled protein is e.coli. Mammalian, yeast and insect cells are used for protein expression when e. coli cannot experss the protein in a useable form. CIL offers a rich array of reagents and products to label e.coli, insect, yeast and mammalian cells in order to produce uniform or selectively labeled protein suitable for NMR experiments.
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