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NMR .

NMR

Solution-state Nuclear Magnetic Resonance (NMR) is a technique that usually requires a sample to be dissolved in a deuterated solvent to reduce the intensity of the solvent resonance.  Both solid-state and solution-state NMR is used to study the structure, function and dynamics of biological polymers such as protein, RNA and DNA.  To get useful signals, these biological molecules typically require enrichment in either 15N or both 13C and 15N.  To get useful signals from  molecules >25 KDa in size, biological molecules also typically require deuterium enrichmenl.
CIL sells a variety of isotope-enriched reagents to label protein, RNA and DNA.  Enriched protein is most often expressed in genetically engineered e. coli cells that are cultured in the presence of stable isotopes.  Yeast, mammalian and insect cells are used to express protein that is unable to be produced in c. coli cells. Cell-free expression methods negate entirely the need of working with living cells and represent the most flexible platform for producing labeled protein.   Enriched nucleic acid is produced in vitro using polymerase chain reaction using with stable isotope enriched nucleotide 5’ triphosphates.  

Amino Acids
Buffers and Reagents
Carbohydrates
Cell Growth Media
Cell-Free Protein Expression
Fatty Acids & Lipids
α-Keto Acids
Methyl Labeling Kits and Amino Acids
MRI/MRS Products
NMR Solvents and Consumables
Nucleic Acids
Protein NMR Standards
Protein Reagents
qNMR Standards
15N Salts
Water

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