Bacterial Media

The over-expression of protein using e. coli is the most popular way to obtain labeled protein for use in NMR studies.  This is achieved by growing e. coli cells and inducing protein expression in a suitably isotope-enriched cell culture medium.  A minimal medium is a cell culture medium that only includes glucose or glycerol or pyruvate as the sole source of carbon and either ammonium chloride or ammonium sulfate as the sole source of nitrogen for the expressed protein. A rich medium contains amino acids as well as some level of glucose for protein production.
CIL offers a number of products for the expression of labeled protein for use in NMR studies. These include reagents for use in minimal media as well as rich bacterial cell media that is ready to use as-is or after dilution with water. CIL also offers Celtone Powder, a labeled additive for use with minimal media as well as Spectra 9 media, a minimal medium that contains 1 gram per liter of Celtone Powder.  CIL also sells e. coli OD media that is produced by Silantes Gmbh, a company located in Munich, Germany.