Buffers, Reagents, Lipids and Detergents
It is best to simply use a deuterated buffer instead of a protonated buffer to reduce the contribution of proton signals from the buffer in the 1H-NMR spectrum from a sample containing a buffer. The buffering capacity is not affected by the presence of deuterium. Likewise, the best way to reduce proton signals in 1H-NMR from the use of common reagents is to use deuterated reagents, including such reagents as deuterated acids, bases and reducing agents.
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