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Reductive Methylation Reagents

Dimethyl labeling is a fast, specific, and inexpensive means to quantify proteins in different sample types (e.g., biofluids and tissues) for variable purposes (e.g., protein expression profiling, quantitative analysis of post-translational modifications).  In this approach, combinations of dimethyl reagents (formaldehyde and cyanoborohydride in their stable isotope-labeled and/or unlabeled form) are added to a peptide mixture before downstream LC-MS analysis.  These reagents label the peptides at their primary amines with isotopomeric tags enabling multiplexed sample measurement in each MS analysis.  CIL offers a number of stable isotope-labeled and unlabeled dimethyl reagents for reductive amination in MS-based quantitative proteomics.

For additional information on this quantitative approach and the accompanying reagents, please refer to our Dimethyl Labeling application page as well as to our Stable Isotope Standards for Mass Spectrometry catalog. 


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